| |
Abstract/Syllabus:
|
Sharp, Phillip, and Richard Young, 7.60 Cell Biology: Structure and Functions of the Nucleus, Spring 2006. (Massachusetts Institute of Technology: MIT OpenCourseWare), http://ocw.mit.edu (Accessed 08 Jul, 2010). License: Creative Commons BY-NC-SA
Graphic illustration of a cell nucleus. (Image courtesy of the U.S. National Library of Medicine.)
Course Highlights
This course features a bibliography of readings.
Course Description
This course covers the fundamentals of nuclear cell biology as well as the methodological and experimental approaches upon which they are based. Topics include Eukaryotic genome structure, function, and expression, processing of RNA, and regulation of the cell cycle. The techniques and logic used to address important problems in nuclear cell biology is emphasized. Lectures cover broad topic areas in nuclear cell biology and class discussions focus on representative papers recently published in the field.
Syllabus
Course Description
The goal of this course is to teach both the fundamentals of nuclear cell biology as well as the methodological and experimental approaches upon which they are based. Class meetings at the start of the week will generally be lectures describing the background and fundamental findings in a particular area of nuclear cell biology. The second session class meetings of the week will generally be a group discussion of one assigned paper in the same area. These seminal papers are used to understand both the important elements of the field as well as to provide concrete examples of the experimental approaches and logic used to establish these findings. Two students will be assigned to present the paper for each discussion based class and faculty and students will participate in the discussion. All students will be expected to have read the paper under consideration and be prepared for a lively discussion.
Enrollment is open to all biology graduate students and undergraduates by permission.
Evaluation
Students will be evaluated by two criteria. Participation in class discussions will constitute 50% of the grade. The remaining 50% of the evaluation will be based on a written proposal due at the end of the semester.
Grading criteria.
ACTIVITIES |
PERCENTAGES |
Class Discussions |
50% |
Written Proposal |
50% |
Calendar
PAS = Prof. Phillip Sharp
RAY = Prof. Richard A. Young
Course schedule.
SES # |
TOPICS |
LECTURERS |
1 |
Introduction: Nucleus |
RAY |
2 |
Lecture: Genome |
RAY |
3 |
Discussion: Genome |
|
4 |
Lecture: Cell Cycle Control |
RAY |
5 |
Discussion: Cell Cycle Control |
|
6 |
Lecture: Transcription Apparatus and Regulators |
RAY |
7 |
Discussion: Transcription Apparatus and Regulators |
|
8 |
Lecture: Chromatin and Gene Expression I |
PAS |
9 |
Discussion: Chromatin and Gene Expression I |
|
10 |
Lecture: Chromatin and Gene Expression II |
RAY |
11 |
Discussion: Chromatin and Gene Expression II |
|
12 |
Lecture: Genome Replication/Repair |
Guest Lecturer: Prof. Angelika Amon |
13 |
Discussion: Genome Replication/Repair |
|
14 |
Lecture: Nuclear Structure, Import/Export |
PAS |
15 |
Discussion: Nuclear Structure, Import/Export |
|
16 |
Lecture: DNA Modification/Epigenetics |
Guest Lecturer: Prof. Rudy Jaenisch |
17 |
Discussion: DNA Modification/Epigenetics |
|
18 |
Lecture: Alternative Splicing/Polyadenylation |
PAS |
19 |
Discussion: Alternative Splicing/Polyadenylation |
|
20 |
Lecture: Signal Transduction |
RAY |
21 |
Discussion: Signal Transduction |
|
22 |
Lecture: RNA Interference I - Silencing and microRNAs |
PAS |
23 |
Discussion: RNA Interference I - Silencing and microRNAs |
|
24 |
Lecture: RNA Interference II - Transcription |
PAS |
25 |
Discussion: RNA Interference II - Transcription |
|
26 |
Course Review: Group Discussion |
|
|
|
|
Further Reading:
|
Readings
Course readings.
SES # |
TOPICS |
READINGS |
3 |
Discussion: Genome |
Lindblad-Toh, K. et al. "Genome sequence, comparative analysis and haplotype structure of the domestic dog." Nature 438 (2005): 803-819. |
5 |
Discussion: Cell Cycle Control |
Malumbres, M., R. Sotillo, D. Santamaria, J. Galan, A. Cerezo, S. Ortega, P. Dubus, and M. Barbacid. "Mammalian cells cycle without the D-type cyclin-dependent kinases Cdk4 and Cdk6." Cell 118 (2004): 493-504. |
7 |
Discussion: Transcription Apparatus and Regulators |
Spilianakis, C.G., Lalioti, M.D., Town, T., Lee, G.R., and Richard A. Flavell, R.A. "Interchromosomal associations between alternatively expressed loci." Nature 435 (2005): 637-645. |
9 |
Discussion: Chromatin and Gene Expression I |
Schmitt, S., M. Prestel, and R. Paro. "Intergenic transcription through a polycomb group response element counteracts silencing." Genes Dev 19 (2005): 697-708. |
11 |
Discussion: Chromatin and Gene Expression II |
Carrozza, M.J. et al. "Histone H3 Methylation by Set2 Directs Deacetylation of Coding Regions by Rpd3S to Suppress Spurious Intragenic Transcription." Cell 123 (2005): 581-592. |
13 |
Discussion: Genome Replication/Repair |
Uhlmann, F., D. Wernic, M. A. Poupart, E. V. Koonin, and K. Nasmyth. "Cleavage of cohesin by the CD clan protease separin triggers anaphase in yeast." Cell 103 (2000): 375-386. |
15 |
Discussion: Nuclear Structure, Import/Export |
Gerber, A. P., D. Herschlag, and P. O. Brown. "Extensive association of functionally and cytotopically related mRNAs with Puf family RNA-binding proteins in yeast." PLos Biol 2 (2000): E79. |
17 |
Discussion: DNA Modification/Epigenetics |
Mager, J., Montgomery, N.D., Pardo-Manuel de Villena, F., and Magnuson, T. "Genome imprinting regulated by the mouse Polycomb group protein Eed." Nature Genetics 33 (2003): 502-507. |
19 |
Discussion: Alternative Splicing/Polyadenylation |
Han, K., G. Yeo, P. An, C. B. Burge, and P. J. Grabowski. "A combinatorial code for splicing silencing: UAGG and GGGG motifs." PLoS Biol 3 (2005): E158. |
21 |
Discussion: Signal Transduction |
Simone, C., Forcales, S.V., Hill, D.A., Imbalzano, A.N., Latella, L., and Puri, P.L. "p38 pathway targets SWI-SNF chromatin-remodeling complex to muscle-specific loci." Nature Genetics 36 (2004): 738-743. |
23 |
Discussion: RNA Interference I - Silencing and microRNAs |
Stark, A., J. Brennecke, N. Bushati, R. B. Russell, and S. M. Cohen. "Animal MicroRNAs Confer Robustness to Gene Expression and Have a Significant Impact on 3'UTR Evolution." Cell 123 (2005): 1133-1146. |
25 |
Discussion: RNA Interference II - Transcription |
Verdel, A., S. Jia, S. Gerber, T. Sugiyama, S. Gygi, S. I. Grewal, and D. Moazed. "RNAi-mediated targeting of heterochromatin by the RITS complex." Science 303 (2004): 672-676.
Sugiyama, T., H. Cam, A. Verdel, D. Moazed, and S. I. Grewal. "RNA-dependent RNA polymerase is an essential component of a self-enforcing loop coupling heterochromatin assembly to siRNA production." Proc Natl Acad Sci 102 (2005): 152-157. |
26 |
Course Review: Group Discussion |
|
|
|
|
Rating:
0 user(s) have rated this courseware
Views:
29404
|
|
|
|
|