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 Experimental Methods of Adjustable Tetrode Array N  posted by  duggu   on 1/22/2008  Add Courseware to favorites Add To Favorites  
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Abstract/Syllabus:

Wilson, Matthew, 9.96 Experimental Methods of Adjustable Tetrode Array Neurophysiology, January IAP 2001. (Massachusetts Institute of Technology: MIT OpenCourseWare), http://ocw.mit.edu (Accessed 08 Jul, 2010). License: Creative Commons BY-NC-SA
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Photograph of a tetrode array.

The tetrode array, used to record from multiple neurons in the brain, is the final project of the class. (Image by Prof. Matthew Wilson.)

Course Highlights

The course is a hands-on experimental laboratory class with some preparatory lectures and descriptive procedural handouts. Teams of students will embark an intensive effort to execute neurophysiological experiments from start to finish. This will include manufacturing and construction of the implanted recording and stimulation microdrive array, animal training, experiment preparation, running of behavioral experiments, and subsequent data post-processing. There will be opportunities to participate in all phases of the experimental process.

This course is offered during the Independent Activities Period (IAP), which is a special 4-week term at MIT that runs from the first week of January until the end of the month.

Course Description

Students will be exposed to all aspects of a cutting-edge technique in modern electrophysiology, in a highly structured, team oriented environment. The research projects will probe the neural mechanisms of learning and memory through tetrode array recordings coupled with patterned microstimulation. Due to the broad nature of tasks to be completed, coupled with the team oriented approach we will be employing, we are interested in students with a wide variety of laboratory experience and skill levels.

Syllabus

 
Overview

In this class, we will execute the entire process of rodent behavioral neurophysiology experiments - from construction of the implanted microdrives through experiment performance and data analysis. Students will be exposed to all aspects of a cutting-edge technique in modern electrophysiology, in a highly structured, team oriented environment. The research projects will probe the neural mechanisms of learning and memory through tetrode array recordings coupled with patterned microstimulation.

Introduction

Our success as a team and as individuals in this endeavor relies on the quality of everyone’s work, which is a direct reflection of your work habits. Every step in the process relies on the proper completion of all previous steps. Remember to keep your quality standards high at all times. Defects in workmanship will propagate down the production line, forcing you to retrace your steps from that point on. If it ain’t right – toss it. Materials are cheap. Time is costly.

Likewise, good work habits will lead directly to quality work. Please keep the lab clean and organized. Clean up your work areas frequently, and put things away where they belong when you are done with them. The last fifteen minutes of every class will be devoted to lab cleanup and review of daily progress. We have a lot of people working in a very small space, and using a lot of shared equipment. Only through cleanliness, organization and common courtesy will it work for all of us.

That being said, I think that this month should be interesting and a lot of fun. I hope to maintain a productive, exciting and collegial atmosphere throughout IAP and beyond. When we all work together, who knows what we will be able to achieve?

Project

1. Produce a functioning tetrode array and microdrive assembly.
2. Record from the brain of a behaving animal.

Toolkit Inventory List

Each team should have a toolkit consisting of the following items:

1. Tool Box (black)
2. Drive Parts Box (clear)
3. Pana-Vise for holding drive as it is being built and loaded
4. Scribe
5. Exacto-Knife
6. Large Pliers
7. Fine Pliers
8. Super Glue (2)
9. 1 ml Syringes (3)
10. 26 Gauge Needles (3)
11. Fine Scissors
12. Diagonal Cutters
13. Tetrode Tweezers
14. 0-80 Screws (100)
15. 0-80 Screw Taps (2)
16. Tap Handle
17. Tetrode Box
18. Tetrode Stripper (burner)

Calendar

 

Lecture 1 Introductory Lecture

"The Hippocampal Formation and its Role in Memory – Investigations through Tetrode Microarray Recording"

Project Orientation

  • Project Description
  • Strategy Description
  • Team Formation and Intra-job Delegation

Lab Orientation and Task Overview

  • Animal Training
  • Drive Building
  • Drive Loading & Tetrode Manufacturing
  • Paradigm Apparatus Construction

Lecture 2 "Detailed Laboratory Task Training"

  • Animal Training
  • Drive Building
  • Tetrode Manufacturing
  • Paradigm Apparatus Construction

Lecture 3

  • Animal Training – Handling
  • Drive Building – Drive parts
  • Tetrode Manufacturing – 30 uncut (per team)
  • Paradigm Apparatus Construction – 8 arm and extra linear track in progress

Lecture 4

  • Animal Training – Linear track
  • Drive Building – Drive parts
  • Tetrode Manufacturing – 40 uncut (per team)
  • Paradigm Apparatus Construction – 8 arm and extra linear trackin progress

Lecture 5 "Advanced Microdrive Piece Construction"

  • Animal Training – Linear track
  • Drive Building – Base
  • Tetrode Manufacturing – 40 uncut (per team)
  • Paradigm Apparatus Construction – 8 arm and extra linear track in progress

Lecture 6

  • Animal Training – Linear track
  • Drive Building – Screws
  • Tetrode Manufacturing – 30 burnt
  • Paradigm Apparatus Construction – 8 arm and extra linear track in progress

Lecture 7 

  • Animal Training – 8 arm and linear track
  • Drive Building – Screws
  • Tetrode Manufacturing – 40 burnt
  • Paradigm Apparatus Construction – 8-arm and extra linear track in finished

Lecture 8 "Microdrive Assembly"

  • Animal Training – 8 arm and linear track
  • Drive Building – Head stage and assembly
  • Tetrode Manufacturing – 40 burnt

Lecture 9 "Microdrive Preparation and Optimization"

  • Animal Training – 8-arm and linear track
  • Drive Building – Drive at optimum functionality

Lecture 10 "Techniques of Drive Loading, Testing and Preparation"

  • Animal Training – 8-arm and linear track
  • Drive Loading –1st section loaded (check for shorts and conductivity)

Lecture 11

  • Animal Training – 8-arm and linear track
  • Drive Loading – 2nd & 3rd section loaded (check for shorts and conductivity)

Lecture 12

  • Animal Training – 8-arm and linear track
  • Drive Loading

Lecture 13 "Rodent Neurosurgery Procedures & Protocol"

  • Neurosurgery

Lecture 14

  • Neurosurgery

Lecture 15 "Experimental Protocol"

  • Animal Care
  • Experiment Execution

Lecture 16

  • Animal Care
  • Experiment Execution

Lecture 17

  • Animal Care
  • Experiment Execution & Data Analysis

Lecture 18

  • Animal Care
  • Experiment Execution & Data Analysis

Lecture 19

  • Animal Care
  • Experiment Execution & Data Analysis
  • Course Wrap-up and Overview
 



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